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Asparagus tissue culture basic equipment
To carry out tissue culture, a specialized laboratory is essential, consisting of several key areas such as the washing room, sterilization room, preparation room, aseptic operation room, culturing room, identification room, and a shaded nursery shed. Each section has a specific function to ensure the success of the tissue culture process.
The **washing room** is primarily used for cleaning glassware, tools, and materials. It should be equipped with sinks, buckets, wash basins, hoses, test tubes, workbenches, shelves, drying ovens, and waste bins. This area is typically connected to or located near the preparation room for convenience.
The **sterilization room** is responsible for sterilizing culture media, instruments, and using bacterial filters. Essential equipment includes autoclaves (both portable and upright), electric or gas stoves, and carts. The room is usually adjacent to the preparation room and the aseptic operation room to maintain sterility.
The **preparation room** is a critical space for storing reagents, chemicals, and preparing culture media. It should have refrigerators, storage cabinets, scales, various reagents, glassware, pipettes, test tube racks, pH meters, and microscopes if possible. This room serves as the central hub for material preparation before moving to the sterile environment.
The **aseptic processing room**, also known as the inoculation room, is where all sterile operations take place. It must have a laminar flow hood or a clean bench, along with UV lamps for disinfection. Typically, this room is separated from the outside by a buffer zone with sliding doors to prevent contamination. A UV lamp and a hanger are also installed in the buffer area for additional cleanliness.
The **culturing room** is where plant tissues grow, forming callus, seedlings, and developing into healthy plants. This room requires good insulation, ventilation, and lighting. It should be close to the aseptic area and equipped with air conditioning, cooling systems, and artificial lighting that mimics natural conditions. Temperature control devices, such as thermostats, are necessary to maintain optimal growth conditions. Cold light lamps are preferred for illumination, while incandescent bulbs can be used in colder seasons for warmth. The room structure should be sturdy, with multi-tiered shelves for efficient use of space.
Finally, the **transitional cultivation room or shed** is where rooted plantlets are moved from test tubes into soil-like substrates such as vermiculite, perlite, or small pots filled with nutrient-rich soil. This step is crucial for acclimating the plants to field conditions. The environment in this area should be carefully controlled in terms of temperature, light, and humidity to increase the survival rate of the seedlings. Proper management during this phase ensures a successful transition from the lab to the field.