The Artificial Breeding Techniques of C. edulis

1. Breeding of broodstock Broodstock cultivation is a basic link in the artificial propagation of the head lice. The quality of broodstock cultivation directly affects the maturity of the gonad, the rate of inoculation, the fertilization rate and the hatching rate of the eggs. Therefore, in the daily work, the work of broodstock breeding should be done, done fine and done.
Basic requirements: Whether it is artificial breeding or natural growth of the head lice, as long as the sexual maturity (usually 2-3 years) weight 400-500 grams or more, good physical condition, no disease, no phytotoxicity, then in a good environment Under aquaculture conditions, there are sufficient foods to ensure the supply of necessary nutrients, and they can be cultivated into broodstock that can supply oyster production. Therefore, the method of broodstock breeding must be adapted to the basic rules of gonadal development so as to cultivate good quality broodstock with good maturity.
(1) Sexual maturation and sex cycle The head of a sexual mature age is 400-500 grams or more of the general body weight. The ovaries degenerate into stage II from July to October, and from stage III to stage IV from the beginning of April to the beginning of the following year. It begins to develop into stage IV in April. The ovarian maturation coefficient is up to 23.7% in the middle of May to June, and ovarian development maturation coefficient = ovarian weight/ 100% weight. In this case, V period, postpartum ovaries begin to degenerate.
(2) The selection of broodstock and feeding of broodstock can be selected from ponds, but it is best to choose from large groups of lakes, such as lakes and swings. It is better to choose brood fish over 3 years of age and weigh more than 500 grams.
The broodstock can be polycultured or monocultured, and is usually polycultured in brood, oyster, and grass brood fish ponds. In recent years, all good-breeding farms and breeding farms have started to grow monoculture. When broodstock is cultivated, the area of ​​broodstock breeding ponds is 3-5 mu, the water depth is 1.5-1.8 meters, the water pH is 6.5-7.0, and the dissolved oxygen is not less than 5 mg/litre pond. The water quality formed a micro-fluid, with a stocking density of 250-350 tails/mu. The head lice should be fed mainly with green feed, but they must also be fed with plant-based concentrates such as soybean meal and wheat bran. During the breeding season and postpartum, the amount of concentrated feed should be increased. The amount of feed should be 2%-3% of the fish's body weight to promote the development of gonadal development or postpartum recovery.
(3) Discrimination of male and female males The fins and pelvic fins of the head, pectoral fins, and caudal peduncles of the male reproductive culprit during the reproductive season have a dense "chasing star". The first fins of the pectoral fins are hypertrophic and wavy. This characteristic will not disappear for a lifetime. , light pressure on the abdomen with milky semen outflow. Except for the tail shank of females, the rest of the females had almost no "chasing star." The first fins of the pectoral fins were fine and straight, and the abdomen was obviously enlarged and soft, and the salivation was slightly prominent. The broodstock approaches the spawning season in March each year. When the water temperature rises to 16°C, the male and female broodstock should be bred in order to avoid weather changes such as storms. As the weather becomes clear and the water temperature rises, the broodstock needs to be produced and the production plan is seriously out of control. Avoid unnecessary losses.
2. The amount of egg-returning is calculated according to the ovaries of stage IV. Specifically refers to the number of egg cells that the female fish contains yolk globules. The highest fish was the fourth-instar fish, followed by the third-instar fish, and the highest in the third-instar fish.
3, oxytocin hatching
(1) Natural egg-laying group After the gonads have matured, when the water temperature rises above 20°C, select sunny and windless weather, combine male and female broodstock and pool, and allocate 1:1 ratio of male to female to give appropriate flow. Stimulation and timely placement of fish nests (poplar poplars, poplars, or 70-mesh sieves into fish nests) can spawn naturally.
(2) Artificial oxytocin production At present, artificial oxytocin production methods are commonly used in production so that broodstock can concentrate on spawning. Pituitary gland, chorionic hormone and releasing hormone analogues have oxytocin effect. The oxytogen dose is 6-8 mg/kg in the pituitary gland, 1 000-1 500 IU/kg of chorionic hormone, 60-100 μg/kg of hormone analogues, and half the dose of male fish. Generally, one-time injection is used. The best injection time is between 17:00 and 18:00. Control the spawning around the dawn of the following day, the water temperature is 22-24°C, and the effect time is between 8:30-9:30.
(3) After hatching 1 self-produced broodstock is injected with oxytocin, it can be hatched with a fry cultivation pond. That is, the pre-treated pool water level is controlled to be above 0.8 meters, the pond is 2 meters away from the pool, and a fish nest is suspended to form a frame. A poplar root or a 60-mesh sieve is used to make the spawning fish nest. After the fish nest is laid out, choose sunny and suitable temperature, and incubate the broodstock that has been injected with the oxytocide and incubate it with microfluidic water to allow it to spawn on its own. After production, the fish nests with eggs are put into a special hatching pond for hatching, and 250,000-300,000 pieces of eggs are laid per acre, allowing them to emerge on their own. If there is continuous rain, the water temperature is low, you can use potassium permanganate 2% solution into the pool after bathing, to prevent the mold of fish eggs after entering the pool, seriously affecting the emergence rate.
2 head lice artificial insemination method. When the natural spawning is affected by the weather, spawning is not much, and the emergence rate is low, use oxytocin. Occupation time is too long, through artificial oxytocin and artificial insemination, can promote egg maturation, spawning, and neat emergence. The dose requirement for oxytocin is not strict for the bolus bolus, but the oxytocide requires a uniform dose. The oxytocin, a commonly used oxytocin, is effective for oxytocin production in the pituitary, chorionic hormone, and hormone analogs, and can be used singly or in combination. When used, consideration should be given based on the conditions of the fish and the environmental conditions. For example, in the low temperature stage, LRH can be mixed with 5 μg/tail, and fish with poor gonadal development can also be mixed with LRH.
3 injection method First, the pectoral fin base abdominal injection, the right hand holding the syringe, the index finger stretched against the needle, leaving 1 cm to control the depth of the needle, the syringe up the scale, in order to grasp the output of the injection, head to the fish Left, with his left thumb to open the pectoral fins, the right hand is to the pectoral fin base needle, his left hand pectoral fins, his right hand with the index finger and middle finger grip the back end ring of the syringe, the thumb quickly push the syringe core, the injection is carried The oxytocide is slowly injected into the fish.
Second, the back muscle injection, the same method to hold the syringe, the right hand with a needle to the scales dorsal fin above the scales provoke the scales, so that the needle piercing the muscle straight down into the vertical angle, slowly push into the injection. If the muscles appear bulging, the left hand can be used for massage, so that the oxytocreas gradually spread to achieve effects. When injecting oxytocic agents, the progeny of fish leaps and should be protected by another person. In the protection, cotton products and prefabricated pouches are used as protection tools to protect the broodstock from traumatic injury, so as to facilitate the operation and achieve the purpose of spontaneous delivery.
Third, the effluent effect time. The effect of the bolus of bolus oxytocin is related to water temperature. 22-26°C is its optimum spawning temperature and its effect time is 8-12 hours. The oxytocin boluses are also used in the second injection, that is, the first shot is 1/3 of the total dose of the oxytocin by the female fish, and the second shot is the full amount after 8-12 hours. This method is not suitable for use in mass production, and is not used for sexual maturity. It is only used when the development of the population is not synchronized or artificially inseminated.

Best Goji procuct is in China,China is the origen of Goji Berry.Goji is  a traditional,typical food in China.The majority of commercially produced goji comes from Ningxia and Xinjiang in Northwestern China, where they are grown on plantations totaling 200,000 acres.[self-published source] In Zhongning County, Ningxia, wolfberry plantations typically range between 40 and 400 hectares (100–1000 acres or 500–6000 mu) in area. As of 2005, over 10 million mu have been planted with goji in Ningxia.

Ningxia goji has been cultivated along the fertile floodplains of the Yellow River for more than 700 years and have earned a reputation throughout Asia for premium quality sometimes described commercially as "red diamonds". Government releases of annual wolfberry production, premium fruit grades, and export are based on yields from Ningxia, the region recognized with-the largest annual harvest in China, accounting for 45% of the nation's total yield of wolfberries (50,000 tons, 2013);
formation of an industrial association of growers, processors, marketers, and scholars of wolfberry cultivation to promote the berry's commercial and export potential;[16]
the nation's only source of superior grade wolfberries used by practitioners of traditional Chinese medicine.

Ningxia Dried Goji Berry

Ningxia Dried Goji Berry,Ningxia Goji Berry,Dried Goji Berry,Organic Ningxia Dried Goji Berry

Ningxiahong Goji Industry Group Company Limited , https://www.ningxiahonggroup.com