Production of trehalose with corn starch

Trehalose is a stable, non-reducing disaccharide widely found in algae, bacteria, fungi, yeasts, insects, and invertebrates. It is not only a storage carbohydrate, but also an important product of stress metabolism. It is also a non-reducing sugar made up of special disaccharide molecules. Its characteristics are very stable, and it can be under severe conditions such as high temperature, high cold, dry and water loss, etc. A special protective film is formed on the surface of cells to effectively protect the structure of biomolecules from being destroyed, so as to maintain the life processes and biological characteristics of living organisms, and can be widely used in various industries such as biological preparations, medicines, foods, cosmetics, and agricultural sciences.
In foods and medicines, it can protect biofilms and biomacromolecules very effectively, avoiding the cells from being damaged by drying, freezing and osmotic pressure changes; it can effectively protect DNA from radiation-induced damage; it can be used as a substitute for medicine. Plasma protein is used as a stabilizer for biological products and vaccines; it is used in cosmetics and has a moisturizing function; it is used in food preservation and food sweeteners and can replace high-caloric sucrose, especially suitable for diabetics; it is used in children's foods such as candy, Chocolate sweeteners can effectively reduce the incidence of dental caries in children.
It is understood that so far, the world's large-scale production of trehalose companies only Japan's Linyuan Chemical Company and China Nanning, China Connaught Sino Biological Engineering Co., Ltd. At present, the Beijing Technology Exchange Center has completed all small pilot production processes using corn starch as raw materials and enzymatically preparing trehalose, and has met the conditions for pilot testing. Fermentation rate and raw material conversion rate are higher in the production process, which also greatly reduces the cost.
At present, the demand for trehalose in the international market is very large. It is estimated that the annual demand in the next few years will reach 150,000 tons or more. At present, the comprehensive productivity of the international trehalose is only about 80,000 t/year, with the application field. With rapid expansion, the demand for trehalose will also increase correspondingly. The trehalose used in the country was basically imported from Japan. Therefore, there is still a lot of space for enzymatic production of trehalose products instead of imported products. The strains used to produce trehalose from corn starch are non-genetically engineered bacteria. The product is a non-genetically engineered product. There are 20 million newborns each year in China, which needs 1 billion vaccines and trehalose as a stabilizer. The annual production value of this item alone can reach 80 million yuan.
In terms of preservation of food, agricultural products and seafood, with the exception of some companies with lower production levels and not using trehalose, the market demand in 2002 has reached more than 2,500 tons. By 2005, the annual demand for trehalose in China will reach 10 More than ten thousand tons. Therefore, the sale of trehalose is only positioned in the popular food preservation and cosmetics industry, and the revenue is considerable. It is estimated that a production plant with an annual production capacity of 100 tons of trehalose will require an equipment investment of 3 million yuan, a direct raw material cost of about 7,000 yuan/t, a product market price of 79,000 yuan/t, and an annual profit of 4.8 million yuan.
Author: Beijing Technology Exchange Promotion Center

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