Pollution-free breeding of muddy seedlings

DB33/ 561-2005 "Pollutionless Loach" is published in parts and is divided into three parts:
Part 1: Technical Specifications for Seed Breeding;
Part 2: Aquaculture technical specifications;
Part 3: Quality and safety requirements.
This section is the first part of DB33/561-2005.
This section was proposed and managed by the Zhejiang Ocean and Fisheries Bureau.
This section drafted by: Zhejiang Provincial Institute of Freshwater Fisheries, Xinchang County Fisheries Technology Promotion Station.
The main drafters of this section: Zhou Zhiming, Zhang Yuming, Hu Tingjian, Huang Xiaohong.
Introduction Muddy is rich in nutrients, delicious in taste, its protein content is up to 21%, and its medicinal effect is remarkable. Therefore, as a nourishing food, loach is more and more popular. Due to overfishing and worsening habitats, muddy resources have been destroyed and natural production has been greatly reduced. Artificial breeding has become the main channel for market supply, and the culture area has developed rapidly both inside and outside the province. In order to standardize the production environment of muddy and nuisance-free culture, promote muddy aquaculture technology, and ensure product quality and safety, this standard is formulated.
Pollution-free loach: Technical specifications for seed breeding
1 Scope This section specifies the environmental requirements of muddy (Misgurnus anguillicaudtus), broodstock requirements, broodstock breeding, seed breeding and other technologies.
This section applies to the breeding of muddy seedlings.
2 Normative references The following documents contain provisions which, through reference in this section, constitute provisions of this section. For dated references, subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the parties to reach an agreement in accordance with this section to study whether the latest version of these documents can be used . For undated references, the latest version applies to this section.
GB 11607 Fishery Water Quality Standard
GB/T 18407.4 Agricultural product safety and quality Environmental requirements for the production environment of pollution-free aquatic products
NY 5051 Harmless Food Freshwater Aquaculture Water Quality
3 Environmental requirements
3.1 The production environment should meet the requirements of GB/T 18407.4.
3.2 The water source requires fresh water, sufficient water, and no pollution sources around. Water quality of the source should meet the requirements of GB 11607.
3.3 Ponds for aquaculture ponds should be selected or constructed in good condition. Cement ponds or tri-soil ponds are preferred. The bottom of cement ponds is required to be covered with loam soil of 15 cm to 20 cm. The bottom of soil pool should not be sandy soil, and the ponds should be surrounded by cement or plastic. Plates are made and the wall height is 100cm. The inlet and outlet openings are surrounded by wire mesh or nylon screens. The screen mesh is 0.15mm (20 mesh).
4 broodstock requirements
4.1 Sources of broodstock The collection of natural loach spawning in the vicinity of the spawning period or the production unit itself will incubate the individual with a clear growth advantage and avoid inbreeding.
4.2 The broodstock is selected from 2 to 3 instars, females weighing 40g to 100g, males weighing 20g to 50g, with typical germplasm characteristics. Requires strong physique, strong activity, no disease and no injury.
4.3 Male and female sexually mature females generally have enlarged abdomen, small and wide pectoral fins, and a blunt fin at the end. The genital hole is large. On the other hand, the male has a small abdomen, large and narrow pectoral fins, and pointed and pointed ends. There are meat bulges on the rear and flat abdomen. Milky white semen flows from the genital pores during extrusion. When observing from the back of the loach, the abdomen should be white, and individuals with white spots on both sides of the abdomen should not be selected.
5 Broodstock breeding
5.1 Stocking Density The broodstock was placed in a culturing pool in a ratio of 1:1 to 2 for intensive cultivation. The stocking density was 6 groups/m2 to 10 groups/m2.
5.2 Feeding plants in feeding and management ponds are fed twice a day in autumn. Animal feed such as animal minced meat, chopped viscera, and fish meal is mainly used, supplemented by a small amount of plant feed such as wheat bran, bean dregs, rice bran, and cakes. Can be fed compound feed, the amount of feeding is 3% to 5% of body weight. In winter and early spring, feed is stopped, and part of the fermented pig manure is added as a top dressing. Starting in late March, it is necessary to seize the warming season, carry out intensive cultivation, and feed high-protein feeds. The feed rate is 5% to 10%.
5.3 The water level in the water quality management pool shall not be less than 40cm, and new water shall be regularly replenished to adjust the water quality so that the water quality is fat and cool, and the transparency is kept at 30cm.
6 Seed Breeding
6.1 Induction
6.1.1 The production season starts from the end of April to mid-May when the water temperature is above 22°C.
6.1.2 oxytocin oxytocin (HCG), the dose for each female injection 500Iu ~ 7OOIu. Reduce the use of males by half.
6.1.3 injection method The injection site to the back muscle or dorsal fin before and after the two sides as well, but also can use abdominal injection method, the injection site to avoid the abdomen midline. Use No. 4 needle, put the hose or plastic tube at the base of the needle, so that the needle exposed lcm ~ 1.5cm, the gauze can be used to wrap the mud when injected, syringe and fish into the 30O angle, the depth of 0.2cm.
6.2 Spawning
6.2.1 Incubation water temperature The oxy-production optimum temperature is 22°C to 28°C, and the optimum water temperature is 25°C. When the water temperature is 22°C, the broodstock can lay eggs after 14 hours. When the water temperature is between 25°C and 28°C, the mature estrus spawns after 10 hours.
6.2.2 Fertilization After the natural fertilization injection, the effect time is 9h ~ 10h, and it is placed in a cage with a size of 150cm200cm60cm. The mesh or brown sheet is placed as an inducer in the cage. Each group contains 50 to 80 groups of broodstock (male to female ratio). 1:2 to 3). After the eggs have been laid, remove the eggs and place them in an incubator. Artificial Insemination When the broiler estrus severely reaches orgasm, it is picked up and gently squeezes the female's abdomen by hand. The male removes the testes from the grind and then adds Galinger's solution to place the eggs and sperm in a short time. Clean porcelain bowls or porcelain bowls in advance and mix gently with feathers to mix eggs and semen. After fully fertilized, add 0.6% to 0.7% physiological saline, rinse clean, and spread the fertilized eggs evenly. The sterilized fish nests are placed in hatching ponds for incubation. The entire fertilization process prevents direct sunlight.
6.3 Incubation
6.3.1 Incubation Methods Fertilized eggs can be incubated in indoor water or in outdoor microfluidic water. Put the fish nests that have been filled with eggs in the bamboo basket and sink them into the water.
6.3.2 Incubation water dissolved oxygen 6mg/L or more, other shall comply with NY 5051 regulations.
6.3.3 Incubation Density The oviposition densities of hatching cylinders are 150104 particles/m3 to 200104 particles/m3.
6.3.4 filming time When the water temperature at 22 °C ~ 25 °C, 40h ~ 44h can be hatched fry, water temperature 25 °C ~ 28 °C, 24h ~ 28h can be hatched fry. About 3 days after the film is formed, when the color of the fry is changed from black to light yellow, it can be removed from the hatching pool and enter the seedling cultivation stage.

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