PC-810 pesticide residue speed meter instruction manual

PC-810 pesticide residue speed tester manual
Pesticides have become an indispensable means of production in agricultural production, but most of the pesticides are toxic, and pesticides are applied to farmland and various crops year after year (eg, food crops, vegetables, fruits, oils, sugars, Tobacco tea, Chinese herbal medicine, etc.) What are the consequences of this in the long run? It has become a common concern. Pesticide residue tester is mainly used for industrial and commercial quality inspection, health and epidemic prevention, agricultural demonstration base, green food base, family agricultural production, fruit and vegetable suppliers and other production and testing departments to monitor pesticide residues in vegetables and fruits. The purpose of monitoring is to: Before harvesting or listing fruits and vegetables, it is quickly detected whether the pesticide residues in fruits and vegetables exceed the standard. When the amount exceeds the standard, the harvesting period should be extended appropriately to degrade the toxicity of pesticides, prevent excessive fruits and vegetables from entering the market, and bring harm to people's life and health. Avoid unnecessary economic losses to the fruit and vegetable production and distribution department.
This unit is a high-tech enterprise specializing in the development of various agricultural related instruments and equipment. The new information and new achievements of the country have been quoted first. Now it has more than ten years of production history, and its technical strength is at the forefront of China. According to the market demand, we have developed a variety of pesticide residue testers with different price points in the PC series. Nowadays, the PC-810 10-channel arbitrary high-intelligent automatic pesticide residue speed measuring instrument has been developed.
Second , the test principle:
Enzyme inhibition method, according to national standard GB/T 5009.199-2003 "Rapid detection of organophosphorus and carbamate pesticide residues in vegetables" and industry standard NY/448-2001 "organic phosphorus and carbamate pesticides on vegetables" Rapid detection method for residual toxicity. According to organophosphorus and carbamate pesticides, it can inhibit the activity of acetylcholine in the insect central and peripheral nervous system, causing the accumulation of acetylcholine in the neurotransmitter, affecting normal conduction, and causing insect poisoning to death. This insect toxicology principle is used in pesticides. Residual detection. If the vegetable extract does not contain organophosphorus or carbamate pesticides or the residual amount is low, the activity of the enzyme is not inhibited, and the substrate added in the test is hydrolyzed by the enzyme, and the hydrolyzed product is reacted with the added developer to produce colour. On the other hand, if the activity of the vegetable extract containing a certain amount of organophosphorus or carbamate pesticide is inhibited or partially inhibited, the substrate added in the test cannot be hydrolyzed by the enzyme or a little partially hydrolyzed. Color change or color change is very small, and the inhibition rate can be calculated by measuring the absorbance value with time by a photometer, and it can be judged that the residue of the organic phosphorus or carbamate pesticide is contained in the vegetable.
It is widely used in the rapid detection of organophosphorus and carbamate pesticide residues in vegetables, fruits, food, tea, water and soil. It is suitable for agricultural testing centers, vegetable growing bases, farmers markets, supermarkets, health departments, and environmental protection. Departments, industry and commerce, schools, hotels and other fields.
Third , technical indicators:
Detection channel: 10 in-machine data storage: 5000 groups
Lower limit of determination: 0.1-3.0 mg/kg Wavelength: 410 ± 1 nm
Inhibition rate: 0-100%* Measurement accuracy: 5%
Transmittance accuracy: ≤2.0% Transmittance repeatability: ≤0.5%
Linearity error: ≤±3% Inhibition rate error value: ≤5%
Inhibition rate repeatability: ≤5% Repeatability error: absorbance (A) ≤ 0.005
Absorbance value range: 0.0500-3.100 Photocurrent drift: ≤1.0%/3min
Use environment: A, temperature: 5 ° C ~ 45 ° C B, humidity: ≤ 85%
Working voltage: 220V±10%/50HZ Sensitivity: potassium dichromate solution ≥3.17×10-3 (A/ug.ml)
Stability: no preheating required. The transmittance (T) value caused by the drift of the photocurrent within 3 minutes does not vary by more than 1.0% (or the absorbance (A) value of 0.005).
Fourth, product features:
1. Ten-channel large-capacity design, multiple samples can be measured at the same time, and the number of test samples can be set at will.
2, SCM intelligent control, full digital circuit design, can be connected with the computer or can be used alone (with pesticide residue detection data system software).
3. Acetylcholinesterase and butyrylcholinesterase reagents can be used at the same time. The national standards and the standards issued by the Ministry of Agriculture can be selected at will.
4, built-in computer numerical control chronograph function (test relative timing difference is less than 0.5 seconds), fully automatic calculation function, application comparison storage function, automatic printing function, test sample quantity selection function.
5, large-screen LCD Chinese display, user-friendly interface, test data is automatically saved, you can save 5000 sets of data, users can view the test history data at any time.
6, equipped with special software, with communication, online, help, measurement, setting, recording, saving and statistical processing, evaluation functions.
7. Equipped with special sample pretreatment equipment and disposable kits.
8, detection speed 3 minutes can be transmitted through the Internet or LAN to send data and reports to achieve data sharing.
9. High sensitivity, the minimum detection limit for pesticides such as methamidophos and dichlorvos is better than the national standard.
10. The signal acquisition part adopts the original imported crystal from Germany, and the sensitivity is four times higher than that of the domestic components. Stable work and long life, up to 100,000 hours.
11, all solid-state light source, stable operation, the life of the light source is up to tens of thousands of hours.
12. The cassette part adopts array type array array circuit, no mechanical moving parts, anti-interference, anti-vibration, stable and reliable performance.
13. The kit develops color, and the sample and time are used less to prevent secondary pollution.
14. Automatically save and print measurement data, check time and date, and turn pages to view, easy to operate.
15, with USB international standard interface, data can be instantly transferred to the computer or automatically print freely switch.
16. Monitoring network information is powerful, including measurement data system and monitoring management system, and implements functions such as data collection, statistics, query, exchange and printing. It can effectively implement remote monitoring management.
17. Provide complete accessory configuration, adopt beautiful and durable aluminum alloy packaging box, which is convenient for users to operate in fixed laboratory or mobile laboratory.
Fifth , the preparation of reagents:
1. Extract: Pour the reagent in the extract reagent bag and dissolve in 510ml distilled water.
2. Substrate: Dissolve by adding 3.1 ml of distilled water to the reagent bottle labeled “Substrate” and store at 0~4 °C.
3. Color developer: Add 32 ml of extract solution to the reagent bottle labeled “developer” to dissolve.
4. Enzyme reagent: Add 3.1ml extract to the reagent bottle labeled “Enzyme” and dissolve it in 0~4°C environment.
Shake evenly before use. Use one bottle to take one bottle, use it after use, and prevent other substances from being contaminated to avoid solution poisoning. Unused reagents can be placed in cleaned reaction bottles at 0~4 °C. save. The reaction bottle can be washed with decontamination powder → rinse with tap water → rinse with distilled water. Do not use acidic washing solution. It is not recommended to wash with detergent. Decontamination powder can be purchased at general grocery store or medical chemical reagent store. Note: Unformulated reagents should be stored frozen at 0 °C.
6. Effective methods to prevent contamination of reagents:
1. Appliance-specific principles: All instruments used to transfer reagents or samples should be dedicated and labeled to avoid cross-contamination caused by mixing.
2, the reagent "only go out" principle: each time the test, the reagents that are sucked (extracted) from the reagent bottle, whether they are used or not, as long as they are sucked out, do not fight back even if they are not used or can not be used. Otherwise, it is easy to contaminate the entire bottle of reagents, causing greater losses, and even affecting all subsequent tests, resulting in erroneous test data.
Seven , pesticide residue testing operations:
1. Sample extraction: Take 1g of fruit and vegetable samples (the leafy vegetables are cut into a sample with a width of about 1 cm, and the root vegetables are taken from a cross-section sample) into a small beaker (plastic bottle in the accessory box), and 5 ml of the extract is added and allowed to stand. After 10 minutes or 2 minutes of shaking extraction, it is filtered, and the clear liquid is taken as the liquid to be tested.
2, control test: take enzyme 0.1ml (100 ul), extract 2.5ml, add to a special reaction bottle, mix, then add 0.1ml of color developer, substrate 0.1ml. Immediately after shaking, transfer to the cuvette, put it into the instrument in time, and press the control test button for the control test.
3. Sample test: Take 0.1ml of enzyme, 2.5ml of test solution, add it to a special reaction bottle, mix and let stand for 10 minutes (time stop timing), add 0.1ml of color developer and 0.1ml of substrate. Immediately transfer to the cuvette after shaking, put it into the instrument in time, and press the sample test button to test.
4. Interpretation of test results: If the enzyme inhibition rate of the test sample exceeds 50 (ie, the inhibition rate reaches 50% or more), it indicates that the pesticide residue toxicity of the sample to be tested may exceed the safety defined standard. It is recommended to check once or pass the gas phase. Further qualitative and quantitative analysis (HPLC/T17331-1998) by instrumental analysis such as chromatography.
5, container cleaning: attached to the various bottles, beakers, pipettes and other containers washed with tap water five times before use, and then washed twice with distilled water,
5. Use after drying.
Eight, instrument operation steps: host power (9v / 1000mA), printer power (5v / 2A), please pay attention to distinguish, to avoid errors.

The original control solution A value is:
×. ×××
Keep the current A value and press the return key.
Re-detect A value and press delete key
(Screen display one)
please choose
Press the test button to test
Press the View button to view previous records
(Screen 2)

Please put the control solution and press the test button.
Channel 1 A=0.000
Status: Ready
Time record: 180 seconds
(Screen display three)

testing. . . please wait
Channel 1 A=×.×××
Status: Testing
Time record: × × × seconds

(Screen 4)

Control solution completed
Please press the [Save] button to save the data.
Please press [Up and Down] to select the number of samples.
(Five-Screen Display) Note: Press End [storage] button and then continuously press the return key twice, and then prompts.
Number of samples selected: 01
After the selection is completed, press the [Test] button.

(screen six)

Put sample 01 to channel 01 and press [Test]
Channel 1 A=0.00 Channel 2 A=0.00
Channel 3 A=0.00 Channel 4 A=0.00
Channel 5 A=0.00 Channel 6 A=0.00
Channel 7 A=0.00 Channel 8 A=0.00
Channel 9 A=0.00 Channel 10 A=0.00
(screen seven)
testing. . . please wait
Channel 1 A=×.×× Channel 2 A=×.××
Channel 3 A=×.×× Channel 4 A=×.××
Channel 5 A=×.×× Channel 6 A=×.××
Channel 7 A=×.×× Channel 8 A=×.××
Channel 9 A=×.×× Channel 10 A=×.××
  (Screen display eight)

Sample detection completed% inhibition rate
Sample 1 ××.×× Sample 2 ××.××
Sample 3 ××.×× Sample 4 ××.××
Sample 5 ××.×× Sample 6 ××.××
Sample 7 ××.×× Sample 8 ××.××
Sample 9 × ×. × × sample 10 × ×. × ×
Please press: [Storage] [Print] [Back]
(Screen 9)
Sample detection completed Inhibition rate % This page has been saved!
Sample 1 ××.×× Sample 2 ××.××
Sample 3 ××.×× Sample 4 ××.××
Sample 5 ××.×× Sample 6 ××.××
Sample 7 ××.×× Sample 8 ××.××
Sample 9 × ×. × × sample 10 × ×. × ×
Please press: [Storage] [Print] [Back]
  (screen display ten)
If you want to query the test results, please press [Query], press [Up, Down] to scroll through the test records, press [Print] to print the page data, and press [Back] to continue the test.
9. Installation and use of the software:
First, copy the “PC Series Pesticide Residue Tester Dedicated Software” file program from the supplied software CD to the computer hard disk, then connect the instrument host to the computer with the supplied data cable to turn on the instrument switch.
1. Install "USB Driver": Open the "PC Series Pesticide Residue Tester Dedicated Software" folder, then double-click the "USB Driver" folder, double-click "Driver", and then click "INSTALL" to install. Follow the on-screen instructions to complete the installation.
2. Install the “Pesticide Detection Program”: Double-click to open the “Pesticide Detection Program” folder, click “Test Program” to start the software. Click "Settings", set the port, and proofread the time and read the time. Then click "Connect", if the instrument communicates successfully with the computer, it will enter the connected state and you can proceed to the next step.
3. Data upload: Click “New Record Number”, it will display how many data are not currently read. If there is unread data, click “Acquisition”, collect the data into the software database, and then click “Export Data”. ", display all the data in the database, you can query, if you want to save the current data, click "Export Excel", select the storage path, save the current data. After confirming that the save is successful, click "Clear Library Record" to delete the data in the database to avoid mixing with the next test data. When finished, click "Exit" to turn off the software and instrument power.
Ten, printer operation method:
To turn on the printer, first press the eject button on the front panel to expose the cabin to the outside of the case, ready for the next step.
1. Replacement of printing paper: Press both ends of the telescopic paper shaft by hand and take off the paper shaft. Place the new paper roll on the paper shaft, press the ends of the telescopic paper shaft with your fingers, put the paper shaft back in place, and confirm that the paper shaft is securely installed. Press the SEL button, the indicator light is off, and the cut paper head is fed into the paper inlet under the head by hand. Press the LF button, the paper will slowly enter the machine head, and the printer will start to feed the paper until the paper is above the machine head. When it is exposed, it should be exposed to a certain length and then press the LF button and SEL button again, and the paper is replaced.
2, ribbon replacement: use both hands to smash the sides of the engine compartment paper exit, open the front panel, you can remove the ribbon cartridge, replace it with a new ribbon cartridge.
XI. Instrument main box configuration list:
1. Pesticide residue tester host: 1 set
2, dedicated software CD 1
3, cuvette: 10
4, host power supply 1
5, printer power supply 1
6, the data line 1
7, manual 1
Twelve, instrument accessory box configuration list:
1. Balance: (0.1g) 1 set
2, a set of reagents 1 set
3, plastic test tube: 10
4, sample extraction bottle: 20
5, buffer reagent bottle 1
6, filter paper: 1 box
7, wash the bottle: 1
8, 镊子: 1
9, cutting knife: 1
10, micro sampler 100ul: 1 set
11, micro sampler 1-5ml 1 set

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