Ultracentrifuge should be regularly repaired

Centrifugation is the use of a powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor to accelerate the sedimentation velocity of the particles in the liquid, separating the sedimentation coefficients and buoyancy-density materials in the sample. Therefore, it is necessary to use a centrifugal machine to generate a strong centrifugal force in order to force these particles to overcome the diffusion to produce a settling motion. Each system inside the centrifuge is carefully maintained and inspected once a year. The suspicious part of the instrument should be checked and cleaned of internal dirt and dust. When it is not used for a long time, it should be energized regularly to allow the oil circulation and refrigeration system to start running to ensure the smooth flow of the pipe. The following describes the maintenance of each system.

1. The drive system of the drive system is the heart of the centrifuge. Any abnormality in any part of the high-speed motor, the shifting gear, the rotary bearing and the sliding bearing will affect the normal operation of the instrument.

The main abnormalities of the drive system are: abnormal sound; bearing burnout, there is resistance feeling when rotating the rotor; dog gear is broken, high-speed rotating shaft is broken; the speed is slow, the vibration is severe, and the speed does not reach the rated speed; The high-speed motor runs for a long time, and the bearing lubricant is dried, forming a thousand grinding and abnormal sound vibration and unstable rotation speed.

High-speed motor runs for a long time, the carbon brush wear is shortened, the compression spring is loose, the carbon brush adhesion is reduced, the resistance between the carbon brush and the commutator (commutator) is increased, and the carbon foam is contaminated, which can form a spark, burn and reverse At the same time, a discharge "beep" sound is emitted, which affects the normal operation of the motor. The short circuit, open circuit and insulation damage of the high speed motor can cause the motor to heat up and not start. Any of the above abnormal conditions should be immediately shut down for maintenance.

2. Vacuum system maintenance The current ultracentrifuge (high-speed centrifuge without vacuum system) mostly uses a mechanical rotary vacuum pump and oil diffusion pump two-stage vacuum system, the vacuum can reach 10-3mmHg, which greatly reduces the friction of the air. The temperature generated by resistance and friction increases. In order to ensure the degree of vacuum in the centrifugal chamber, the frosted surface of the surface should be wiped clean before being placed on the rotating shaft, and then into the centrifugal chamber, and then the vacuum pump is started to evacuate.

In addition, during the long-term operation of the centrifuge, some water will be immersed in the scoop pump to mix with the oil. Therefore, in each inspection, the oil drain switch at the bottom of the vacuum pump should be opened to drain the water, and then the accessory funnel should be inserted into the oil pipe of the oil drain port, and the MR-100 vacuum oil should be added to the oil level observation center mark. If the line height is found to be too dirty and black before draining, it should just be released and replaced.

After the above inspection, it is checked whether the ethylene pipe under the oil sump is full of oil. If oil accumulation is found, the oil mold separator is blocked, so the top of the oil mist separator must be unscrewed, the top cover removed, and the filter removed.

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