Mobile Digital Video Recorders Mobile Digital Video Recorders, also called MDVR, Vehicle DVR, or Mobile DVR,are a kind of device that compresses and stores the video inside and outside the car after the digital-to-analog convertion with advanced video and audio coding and decoding technology. Meanwhile, Mobile DVR for Vehicles supports GPS positioning, entertainment playback, wireless network transmission and so on.
Mobile Digital Video Recorder Functions
Mobile Digital Video Recorder, MDVR, Vehicle DVR, Mobile DVR, Mobile DVR for Vehicles, 4 Channel Mobile DVR SHENZHEN SANAN TECHNOLOGY CO.,LTD , https://www.sanan-cctv.com
The Mobile Digital Video Recorder, which adopts the high-performance H.264 coding standard, and integrates the 3G video capture, GPS global satellite positioning management, wireless transmission, 3G wireless communication technology, video surveillance technology, is the core part of a new generation of the wireless vehicle video surveillance solutions. The (4 Channel) Mobile DVR has powerful audio and video capture, driving data storage and transmission functions, advanced shockproof, dustproof, and heat dissipation(based on the all-aluminum heat dissipation design concept) patented technologies, with three network communication methods optional, and it is suitable for high-speed Sports, severe vibration, unstable power supply, severe interference, dusty and other harsh vehicle mobile environments, and widely used in buses, subways, trains, long-distance buses, taxis, logistics vehicles, private cars, special vehicles (such as cash transport vehicles) and other mobile video surveillance fields.
2. Support 4 channels voice and video synchronous collection, transmission and storage;
3. Support two-way duplex voice intercom;
4. Support both 3G and Wi-Fi internet;
5. Support server video storage, local hard disk, SD card video storage;
6. Support WEB page configuration parameters and real-time video browse;
7. Sensors, CAN data collectors, GPS and other peripherals can be connected via external serial ports;
8. Optimize the transparent transmission of RS232 serial data, built-in driving recorder function;
9. Support vehicle phone and remote monitoring function;
10. Support Hongdian GPS management platform and GPS historical track playback;
In conclusion, MDVR is very powerful and it is very important to choose a suitable MDVR to support the normal operation of the whole Vehicle Surveillance System, and complish the monitor purpose. "Security Anytime Anywhere, Vehicles Surveillance System Specialist" is the slogan of Sanan Technology, please don't hesitate to contact us if you have any demands for the MDVR, we have different MDVRs for you to choose, and we would also be very glad to give you some advice based on our over 10 years industry experience if you need.
Human heat shock protein 60 (HSP60) ELISA kit instruction manual
Human heat shock protein 60 (HSP60) ELISA kit
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-human HSP60 mAb was coated on the plate, the standard and the HSP60 in the sample were combined with the mAb, and biotinylated anti-human HSP60 was added to form an immune complex attached to the plate. Horseradish peroxidase The labeled Streptavidin is combined with biotin, the substrate working solution is blue, and finally the stop solution sulfuric acid is added, and the OD value is measured at 450 nm. The HSP60 concentration is directly proportional to the OD value, and the HSP60 concentration in the sample can be obtained by drawing a standard curve.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 8ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA), cell culture supernatant, tissue homogenate and other lysate products as soon as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 0.5ml of distilled water before use and mix well to form a solution of 16000pg/ml. Set 8 tubes of standard tube, and add 200 ul of standard dilution solution to each tube. Add 1 ul of 16000 pg/ml of the standard solution to the first tube, mix it, and then aspirate 200 ul with the sampler and transfer to the second tube. Repeat the dilution as described above, and aspirate 200 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Specimen activation method
1. Add 450 ul of the sample dilution to a 1.5 ml imported polypropylene tube and add 10 ul of serum or plasma samples.
2. Add 20ul 1 N HCI, cover tightly and mix up and down. Leave at 2-8 ° C for 60 ± 2 minutes.
3. Add 20ul 1 N NaOH, cover tightly, and mix up and down.
4. Use it immediately, or store at -20/-70 °C for 3 days. Multiply the dilution factor by 50 when calculating the result. ( Note: The level of different specimens HSP60 may vary greatly, please flexibly grasp the dilution according to the actual situation)
    5. Cell culture supernatant or tissue homogenate 10-fold dilution (410 ul of sample dilution + 50 ul sample + 20 ul of 1 N HCI + 20 ul of 1 N NaOH).
Test procedure
1. Loading: Add 100 ul of standard or test sample (activated) to each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Take the standard products 8000, 4000, 2000, 1000, 500, 250, 125, 0 pg/ml as the abscissa and OD as the ordinate. Draw on the coordinate paper and draw the standard curve.
3. According to the OD value of the sample, find the corresponding HSP60 content on the graph, and then multiply the dilution factor.
Kit performance
1. Sensitivity: The minimum HSP60 detection concentration is less than 65pg/ml.
2. Specificity: Recombinant or natural human HSP60 can be detected simultaneously. Does not cross-react with other human cytokines.
3. Repeatability: The coefficient of variation in the plate and plate is less than 12%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!