1, DMSO: 2.EB: EB (Ethidium bromide, ethidium bromide) (1) For solutions with an EB content greater than 0.5 mg/ml, treat as follows: (2) A solution having an EB content of less than 0.5 mg/ml can be treated as follows: 3.DEPC: 4. Acrylamide: Any of the following may be classified as subjects of chronic acrylamide poisoning: Treatment principles: 5. NN-methylene bisacrylamide, toxic, affects the central nervous system, do not inhale powder. 6. DTT Dithiothreitol, a strong reducing agent, emits an unpleasant odor. May be harmful to health by inhalation, ingestion or skin absorption. When using solid or high-concentration stock solutions, wear gloves and goggles and operate in a fume hood. 7. TEMED strong neurotoxicity, prevent aspiration, fast operation, and seal when stored. 8. PMSF: Phenylmethylsulfonyl fluoride [(PMSF), C7H7FO2S or C6H5CH2SO2F] is a highly potent toxic cholinesterase inhibitor. It is very damaging to the respiratory mucosa, eyes and skin. It can be fatal due to inhalation, ingestion or skin absorption. Wear suitable gloves and safety glasses, always in a chemical fume hood. In case of contact, immediately flush the eyes or skin with plenty of water, and the contaminated overalls are discarded. 9. Chloroform (CHCl3) is irritating to the skin, eyes, mucous membranes and respiratory tract. It is a carcinogen that can damage the liver and kidneys. It is also volatile and avoids inhaling volatile gases. Wear suitable gloves and safety glasses during operation and always in a chemical fume hood. 10. Formaldehyde (HCOH) is highly toxic and volatile and is also a carcinogen. It is easily absorbed through the skin and has irritation and damage to the eyes, mucous membranes and upper respiratory tract. Avoid inhaling the volatilized volatilization. Wear suitable gloves and safety glasses. Always operate in a chemical fume hood. Keep away from heat, sparks and open flames. 11. Giemsa dyes can be fatal or cause eye blindness and are toxic by inhalation and absorption by the skin. Its possible danger is an irreversible effect. Wear suitable gloves and safety goggles. Operate in a chemical fume hood and do not inhale its powder. 12. Sodium azide (NaN3) is very toxic. It blocks the cytochrome electron transport system. The solution containing sodium azide should be clearly labeled. May be harmful to health by inhalation, ingestion or skin absorption. Wear suitable gloves and safety goggles for extra care. 13. Sodium dodecyl sulfate (SDS) is toxic and is an irritant and poses a risk of serious damage to the eyes. May be harmful to health by inhalation, ingestion or skin absorption. Wear suitable gloves and safety goggles. Do not inhale its powder. 14. Trichloroacetic acid (TCA) is highly corrosive. Wear suitable gloves and safety goggles. 15. Triton X-100 causes severe eye irritation and burns. May be affected by inhalation, ingestion or skin absorption. Wear suitable gloves and goggles. 16. Ammonium persulfate is extremely harmful to mucous membranes and upper respiratory tissues, eyes and skin. Inhalation can be fatal. Wear suitable gloves, safety glasses and protective clothing when handling. Always operate in a fume hood and wash your hands thoroughly after handling. 17. Trizol contains toxic phenol, such as skin contact with Trizol. Please rinse with plenty of detergent and water immediately. If you still have discomfort, please consult your doctor. If there is only a small amount of contact and the symptoms are alleviated after treatment, it is estimated that the problem is not big. HiFent nasal high flow, provide higher flow rate than conventional oxygen treatment during high flow oxygen therapy and process, ideal solution for offering FiO2 that can be manual titration controled, mixed gas with controlled temperature and humidity, so as to achieve better airway management and raise the level of oxygenation. Heated Nasal Cannula,Home High Flow Oxygen,Copd High Flow Oxygen,Oxygen Rate Nasal Cannula Shenyang RMS MEDICAL TECH CO.,LTD , https://www.medicalrms.com
DMSO is dimethyl sulfoxide and is widely used. Used as a solvent for acetylene, aromatic hydrocarbons, sulfur dioxide and other gases, and as a solvent for spinning acrylic fibers. It is an extremely important aprotic polar solvent that is soluble in water and soluble in organic solvents. It has a strong permeability to the skin and helps the drug penetrate into the body. Can also be used as an additive to pesticides. It is also a very important chemical reagent.
DMSO is also a osmotic protective agent, which can reduce the freezing point of cells, reduce the formation of ice crystals, reduce the damage of free radicals to cells, and change the permeability of biofilms to electrolytes, drugs, poisons and metabolites.
However, studies have shown that DMSO has a serious toxic effect, which acts on the protein hydrophobic group, leading to protein denaturation, vascular toxicity and hepatorenal toxicity. DMSO is a relatively toxic substance. Avoid using it when it is used. Prepare 1%-5% ammonia water for use. After skin is applied, wash it with plenty of water and dilute ammonia. The most common are nausea, vomiting, and rash. And it gives garlic, onion, and oyster flavor to the skin and exhaled gases.
Inhalation: High volatile concentrations may cause headaches, dizziness and sedation.
Skin: It can burn the skin and make the skin feel tingling, just like the rash and blisters seen. If dimethyl sulfoxide is in contact with aqueous skin, it will produce a thermal reaction. Avoid contact with dimethyl sulfoxide solutions containing toxic raw materials or substances, because their toxicity is not known, but dimethyl sulfoxide may penetrate into the skin, under certain conditions will be toxic substances into the skin.
Absorption: The risk of absorption is very low.
Ethidium bromide is a highly sensitive fluorescent stain used to observe DNA in agarose and polyacrylamide gels. Ethidium bromide is excited by a standard 302nm UV transilluminator and emits an orange-red signal. The most common method for observing DNA in agarose gel is to use the fluorescent dye ethidium bromide for dyeing. Ethidium bromide contains one that can be inserted into DNA. A tricyclic planar group between the stacked bases. Its binding to DNA has almost no base sequence specificity. In a saturated solution of high ionic strength, one ethidium bromide molecule is inserted approximately every 2.5 bases. When the dye molecule is inserted, its planar group is perpendicular to the axis of the helix and interacts with the upper and lower bases by van der Waals forces. The fixed position of this group and its close proximity to the base results in the fluorescence of the DNA-bound dye, which has a higher fluorescence yield than the dye in the free solution. The DNA absorbs ultraviolet radiation at 254 nm and delivers it to the dye, while the bound dye itself absorbs light radiation at 302 nm and 366 nm. In both cases, the absorbed energy is re-emitted at 590 nm in the red-orange region of the visible spectrum. Since the fluorescence yield of the ethidium bromide-DNA complex is 20-30 times higher than that of the dye without DNA binding, when the gel contains free ethidium bromide (0.5 ug/ml), less can be detected. Up to 10 ng of NDA strips. Ethidium bromide can be embedded in base molecules, resulting in mismatches. Ethidium bromide is a strong mutagen and has high carcinogenicity! Will evaporate at 60-70 degrees (so it is best not to add when the glue is too hot, or should be added to the liquid, 0.5ug / ml, dyed for half an hour) (when EB is added too much, it can also be used at room temperature The stained gel was soaked for 20 min to reduce background fluorescence caused by unbound EB).
Purification of ethidium bromide solution: Since ethidium bromide has certain toxicity, after the experiment is finished, the solution containing EB should be purified and disposed of again to avoid polluting the environment and endangering human health.
1 The EB solution is diluted with water to a concentration of less than 0.5 mg/ml;
2 Add a volume of 0.5mol / L KMnO4, mix, add an equal amount of 25mol / L HCl, mix, set at room temperature for several hours;
3 Add one volume of 2.5mol/L NaOH, mix and discard.
1 Add activated carbon in an amount of 1 mg / ml, gently mix and mix at room temperature for 1 hour;
2 Filter with filter paper and seal the activated carbon and filter paper and discard.
DEPC, diethylprocarbonate, inactivates various proteins and is a strong inhibitor of RNase. DEPC is a potential carcinogen that should be handled under ventilated conditions and avoid contact with the skin. DEPC toxicity is not very strong, but the toxicity of inhalation is the strongest, wearing a mask when using. Inadvertently occupying your hands and paying attention to immediate flushing, RNase AwayTM reagent can replace DEPC, it is easy to operate, low in price, and non-toxic. Simply pour the RNase AwayTM directly onto the surface of the glassware and plasticware, soak it and rinse it off with water to quickly remove the RNase from the surface of the vessel and not interfere with subsequent experiments.
It is a moderately toxic substance. It can enter the human body through skin absorption and respiratory tract. Therefore, protective equipment such as anti-virus clothing, anti-virus masks and anti-virus gloves must be worn during handling and use. The harm of acrylamide is mainly caused by neurotoxicity, as well as reproductive and developmental toxicity. Neurotoxicity is characterized by degenerative changes in peripheral nerves and degenerative changes in the brain involving learning, memory, and other cognitive functions. The trial also showed that acrylamide is a possible carcinogen, and epidemiological observations of occupational exposures indicate that Long-term low-dose exposure to acrylamide can cause symptoms such as lethargy, mood and memory changes, hallucinations, and tremors, along with peripheral neuropathy such as glove-like sensation, sweating, and muscle weakness. Cumulative toxicity, not easy to detoxify.
a. contact with acrylamide on the skin, sweating, dampness, peeling, erythema;
b. There are symptoms such as numbness, tingling, weakness of the lower limbs, and lethargy;
c. Neuro-electromyogram shows suspicious neurogenic damage.
B vitamins, energy mixture can be used, supplemented by physical therapy, physical therapy and symptomatic treatment. Severe poisoning should also strengthen supportive therapy.
Ultraviolet or ultraviolet light can damage the retina of the eye. Never use naked eyes and UV light sources without guards. Commonly used UV sources in the laboratory include portable UV lamps and UV transilluminators. It can only be observed by absorbing filters of harmful wavelengths or safety glass. Ultraviolet light is also a mutagen and carcinogenic. To minimize exposure, ensure that the UV source is properly protected. Wear appropriate preventive gloves when operating under UV light.